tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page  (Bio-Rad)

Journal: Bioactive Materials

Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis

doi: 10.1016/j.bioactmat.2025.11.047

Figure Lengend Snippet: Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). (F) SDS-PAGE analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.

Article Snippet: Hoechst 33342, DAPI solution, Lyso-Tracker Green, Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Live/Dead cell double staining kit, membrane and cytosol protein extraction kit, BCA kit, and SDS-PAGE gel preparation kit were procured from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Zeta Potential Analyzer, Fluorescence, Microscopy, SDS Page, Membrane, Staining, Labeling, Conjugation Assay, In Vitro

Journal: Materials Today Bio

Article Title: Biomimetic cancer cell membrane-coated liposomal nanocarriers loaded with silibinin suppress gastric cancer progression via SNHG1/miR-383-5p/HSP90AA1 axis-mediated PI3K/AKT pathway inhibition

doi: 10.1016/j.mtbio.2025.102744

Figure Lengend Snippet: Construction and characterization of biomimetic nanovesicles CLip@Sil loaded with silibinin and modified with GC cell membranes. Note: (A) Schematic diagram illustrating the synthesis process of CLip@Sil, including extraction of HGC-27 cell membrane proteins, liposome preparation, drug loading, and final assembly, created in BioRender; (B) SDS-PAGE and Western blot analysis of Pan-cadherin, COXIV, and Histone H3 expression in different groups to assess membrane protein purity; (C) IF co-localization showing the overlap between DiR-labeled cell membrane and C6-labeled Lip@Sil signals in CLip@Sil, bar: 50 μm; (D) Particle size distribution of Lip@Sil and CLip@Sil determined by DLS; (E) Zeta potential measurement of both nanovesicle types to assess surface charge characteristics; (F) TEM images of extracted cell membrane, Lip@Sil, and CLip@Sil showing that CLip@Sil exhibits a typical core–shell structure, bar: 100 nm; (G) HPLC analysis of silibinin encapsulation efficiency in Lip@Sil and CLip@Sil; (H) Dialysis-based release study showing silibinin release rates from CLip@Sil under pH 5.0 and pH 7.4 conditions over 24 h. All experiments were performed in triplicate.

Article Snippet: After BCA quantification, 40 μg of protein per sample was loaded onto 10 % SDS-PAGE gels (Cat. No. 4561033, Bio-Rad, USA), followed by membrane transfer.

Techniques: Modification, Extraction, Membrane, SDS Page, Western Blot, Expressing, Labeling, Zeta Potential Analyzer, Encapsulation